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Systematics

Well. DNA fingerprinting of the representative exotic germplasm, comprising 133 samples belonging to thirteen Coffea and 3 Psilanthus species have been done. A large body of DNA typing data has been generated. some of important milestones are here

This work was done at Center for Cellular and Molecular Biology, India..

Phylogenetics of Coffee

Genetic characterization of intra-population variability in founder germplasm of four related species using DNA markers was completed successfully

Defined the parameters for marker based diversity analysis

Established the taxonomic relationships of the coffee species available at CCRI in India

Defined the utility of nuclear ITS domain in sequence based studies to derive the realistic affinities between different Coffea species; need to analyse the whole rather than only one of the variable domain i.e., ITS 1 or ITS 2

The analysis on three different cellular components, nuclear, chloroplast, and mitochondrial regions suggest that organelle DNA may not be ideal candidates for phylogenetic analysis of Coffea species
Completed the fingerprinting of elite arabica germplasm comprising, station-bred selections and rust-differentials,using RAPD, ISSR, AFLP and the ‘in-house” SSR markers; data are being analyzed

Cross-species amplifications using SSR markers suggest Psilanthus species to be distant relatives of Coffea species.