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Transcriptomics
Linkage Map
AFLP Typing - Rice
Microsatellites
Systematics
AFLP Typing - Coffee
MicroPropagation
Apomixis
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The ESTs are single-pass sequences of cDNA clones randomly chosen from a library, they can be used for variety characterization as well as for expression studies. We recovered a total of 2069 ESTs from 3'-end specific cDNA libraries of roots and leaves of C. arabica. The amplification of some of the ESTs on arabica varieties resulted in identification of 3 single nucleotide polymorphic (SNPs), and several heterozygous loci. These SNPs are under development as diagnostic markers...
Coffee, being the beverage of tremendous economic importance, continuous efforts to develop better genotypes is the need of the day. Despite of extreemly limited variation in the arabica gene pool, I have identified a reasonable number of polymorphic microsatellite markers after a tedious and careful screening. The identified markers are being screened for purpose of constructing linkage map. It is predicted to be the first microsatellite based arabica map in the world...
Genetic improvement of coffee, can particularly be triggered using molecular marker approaches. In this context, microsatellites are the small tandem repeat based microsatellite markers that have proven to be most desirable in various genetic studies on germplasm characterization and linkage analysis due to their codominant nature, stability, abundance, sensitivity, ease and speed of analysis, minimal sample requirements and suitability for automation...
I have special interest in molecular systematics. Analysis of both nuclear and extra nuclear DNA sequence has answered some of the basic questions in coffee phylogenetics. In depth phenetic/phylogentic analysis of mobility/sequence based markers have led to the optimization of number of markers/nucleotide sites to be used for such analysis...
Well. DNA fingerprinting of the representative exotic germplasm, comprising 133 samples belonging to thirteen Coffea and 3 Psilanthus species have been done. A large body of DNA typing data has been generated. some of important milestones are...
The cultivated rice is a high volume, low value cereal crop providing staple food to more than 50% of the world populace. A small group of rice cultivars, traditionally produced in Indo-Gangetic plains, popularly known as Basmati, having exquisite quality grain characteristics, are prized commercial commodity. Efforts to improve the yield potential of Basmati have led to development of several crossbred Basmati-like cultivars...
(Pterocarpus santalinus L.) with the ultimate focus on biodiversity conservation. Eleven Red Sanders growing locations were identified all over Tamil Nadu, from which a total of 44 trees were selected based on comparison check tree selection followed by regression analysis. Macropropagation studies were carred out and the protocols for mass multiplication have been sucessfully standardised..
Apomixis, one of the challenging area of research even till today. Accationaly (0.01%), we found twin seedlings (two seedlings from a single seed) in rice nursery. We started collecting such rare/abnormal specimens and trying to find out, why is it happening?. Therefore, there was no definite route to approach the problem. This is like questioning the defence machanism or questioning the evolution of rice. We tried to investigate the problem in different directions. Some of the milestones are.
Transcriptomics
The ESTs are single-pass sequences of cDNA clones randomly chosen from a library, they can be used for variety characterization as well as for expression studies. We recovered a total of 2069 ESTs from 3'-end specific cDNA libraries of roots and leaves of C. arabica. The amplification of some of the ESTs on arabica varieties resulted in identification of 3 single nucleotide polymorphic (SNPs), and several heterozygous loci. These SNPs are under development as diagnostic markers...
Construction of Linkage map
Coffee, being the beverage of tremendous economic importance, continuous efforts to develop better genotypes is the need of the day. Despite of extreemly limited variation in the arabica gene pool, I have identified a reasonable number of polymorphic microsatellite markers after a tedious and careful screening. The identified markers are being screened for purpose of constructing linkage map. It is predicted to be the first microsatellite based arabica map in the world...
Microsatellites
Genetic improvement of coffee, can particularly be triggered using molecular marker approaches. In this context, microsatellites are the small tandem repeat based microsatellite markers that have proven to be most desirable in various genetic studies on germplasm characterization and linkage analysis due to their codominant nature, stability, abundance, sensitivity, ease and speed of analysis, minimal sample requirements and suitability for automation...
Systematics
I have special interest in molecular systematics. Analysis of both nuclear and extra nuclear DNA sequence has answered some of the basic questions in coffee phylogenetics. In depth phenetic/phylogentic analysis of mobility/sequence based markers have led to the optimization of number of markers/nucleotide sites to be used for such analysis...
DNA typing of Indian Coffee
Well. DNA fingerprinting of the representative exotic germplasm, comprising 133 samples belonging to thirteen Coffea and 3 Psilanthus species have been done. A large body of DNA typing data has been generated. some of important milestones are...
DNA Typing of ‘Basmati’ Rice
The cultivated rice is a high volume, low value cereal crop providing staple food to more than 50% of the world populace. A small group of rice cultivars, traditionally produced in Indo-Gangetic plains, popularly known as Basmati, having exquisite quality grain characteristics, are prized commercial commodity. Efforts to improve the yield potential of Basmati have led to development of several crossbred Basmati-like cultivars...
Micropropagation of Red Sanders
(Pterocarpus santalinus L.) with the ultimate focus on biodiversity conservation. Eleven Red Sanders growing locations were identified all over Tamil Nadu, from which a total of 44 trees were selected based on comparison check tree selection followed by regression analysis. Macropropagation studies were carred out and the protocols for mass multiplication have been sucessfully standardised..
Apomixis
Apomixis, one of the challenging area of research even till today. Accationaly (0.01%), we found twin seedlings (two seedlings from a single seed) in rice nursery. We started collecting such rare/abnormal specimens and trying to find out, why is it happening?. Therefore, there was no definite route to approach the problem. This is like questioning the defence machanism or questioning the evolution of rice. We tried to investigate the problem in different directions. Some of the milestones are.
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